이전 바이오텍 카테고리/MolBiol Programs
MASS로부터 단백질의 post-tanslational modification 예측하기
바이오매니아
2007. 3. 19. 23:27
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분자생물학의 발전이 DNA 염기 서열 분석법과 함께 급속도로 발전을 했다면 최근에 질량분석법(MASS spec)의 발전은 단백질체학 및 관련 분야의 발전에 큰 공헌을 하고 있습니다. 보통 단백질을 정제해서 MASS를 찍으면 우리가 계산한 단백질의 분자량과 약간 다르게 나오는 경우가 많은데 그런 경우는 유전자의 변이가 생겼거나 아니면 post-tanslational modification이 일어난 경우일 것입니다.
아래의 사이트는 실제로 얻은 분자량이 이론적인 분자량과 다를 경우 어떤 post-tanslational modification이 일어났는지를 예측할 수 있도록 만들어 놓은 reference들 입니다. 분자량의 차이를 입력하고 확인하실 수도 있고 아니면 밑의 more를 누르시면 전체 reference를 보실 수도 있습니다.
http://www.abrf.org/index.cfm/dm.home
http://www.abrf.org/index.cfm/dm.home?AvgMass=all
| Avg.Mass Change |
Modification |
 | |
| -79 | 5' dephospho |
| -58 | Desmosine (from Lysine) |
| -48 | decomposed carboxymethylated Methionine |
| -44 | decarboxylation of gamma carboxy Glutamate |
| -43 | gamma-glutamyl semialdehyde (from arginine) |
| -42 | Ornithine (from Arginine) |
| -34 | Lysinoalanine (from Cysteine) |
| -34 | Lanthionine (from Cysteine) |
| -34 | Dehydroalanine (from Cysteine) |
| -30 | Homoserine formed from Met by CNBr treatment |
| -27 | Oxidation of arginine (to glutamic acid) |
| -18 | Formylglycine (from cysteine) |
| -18 | Pyroglutamic Acid formed from Glutamic Acid |
| -18 | Dehydration (-H2O) |
| -18 | S-gamma-Glutamyl (crosslinked to Cysteine) |
| -18 | O-gamma-Glutamyl- (Crosslink to Serine) |
| -18 | Serine to Dehydroalanine |
| -18 | Alaninohistidine (Serine crosslinked to theta or pi carbon of Histidine) |
| -18 | Misincorporation of Norleucine for Methionine |
| -18 | Succinimide formation from aspartic acid |
| -17 | Pyroglutamic Acid formed from Gln |
| -17 | N-pyrrolidone carboxyl (N terminus) |
| -17 | N alpha -(gamma-Glutamyl)-lysine |
| -17 | N-(beta-Aspartyl)-Lysine (Crosslink) |
| -17 | Succinimide formation from asparagine |
| -17 | S-carbamoylmethylcysteine cyclization (N-terminus) |
| -16 | Pyruvoyl- (Serine) |
| -5 | Crosslink between Arg and His sidechains |
| -4 | 3,3',5,5'-TerTyr (Crosslink) |
| -2 | Formylglycine (from serine) |
| -2 | Disulphide bond formation (Cystine) |
| -2 | S-(2-Histidyl)- (Crosslinked to Cysteine) |
| -2 | S-(3-Tyr) (Crosslinked to Cysteine) |
| -2 | 3,3'-BiTyr (Crosslink) |
| -2 | IsodiTyr (Crosslink) |
| -1 | Allysine (from Lysine) |
| -1 | Amide formation (C terminus) |
| -1 | Oxidation of lysine (to aminoadipic semialdehyde) |
| 1 | Deamidation of Asparagine and Glutamine to Aspartate and Glutamate |
| 1 | Citruline (from Arginine) |
| 2 | Cysteine x2, reduction of Cystine (Cys-Cys) |
| 2 | Reduction of indole double bond of Trp |
| 4 | Oxidation of Trp to kynurenine |
| 12 | Lysine epsilon amino to imine |
| 12 | Cysteine (N-term) formaldehyde adduct (Cys to Thioproline conversion) |
| 12 | Formaldehyde adduct of Trp |
| 13 | Syndesine (from Lysine) |
| 13 | CM-Cys vs PAM-Cys |
| 14 | Methylation (N terminus, N epsilon of Lysine, O of Serine, Threonine or C terminus, N of Asparagine) |
| 14 | CAM-Cys vs PAM-Cys |
| 15 | delta-Hydroxy-allysine (from Lysine) |
| 15 | Oxidation of lysine (to aminoadipic acid) |
| 16 | Hydroxylation (of delta C of Lysine, beta C of Tryptophan, C3 or C4 of Proline, beta C of Aspartate) |
| 16 | Oxidation of Methionine (to Sulphoxide) |
| 16 | 3,4-Dihydroxy-Phenylalanine (from Tyrosine) (DOPA) |
| 16 | Oxohistidine (from histidine) |
| 16 | Sulfenic Acid (from Cysteine) |
| 16 | Oxidation of proline (to gamma-glutamyl semialdehyde) |
| 22 | Sodium |
| 28 | Ethyl |
| 28 | N,N dimethylation (of Arginine or Lysine) |
| 28 | 2,4-BisTrp-6,7-dione (from Tryptophan) |
| 28 | Formylation (CHO) |
| 30 | 6,7 Dione (from Tryptophan) |
| 32 | 3,4,6-Trihydroxy-Phenylalanine (from Tyrosine) (TOPA) |
| 32 | 3,4-Dihydroxylation (of Proline) |
| 32 | Oxidation of Methionine (to Sulphone) |
| 32 | Oxidation of proline (to glutamic acid) |
| 32 | Double oxidation of Trp |
| 34 | 3-Chlorination (of Tyrosine with 35Cl) |
| 36 | 3-Chlorination (of Tyrosine with 37Cl) |
| 38 | Potassium |
| 42 | Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) |
| 42 | N-Trimethylation (of Lysine) |
| 43 | Carbamylation |
| 44 | disodium |
| 45 | Nitro (NO2) |
| 46 | beta-Methylthio-aspartic acid |
| 48 | Cysteic acid, oxidation of cysteine |
| 51 | Piperidine adduct to C-terminal Cys |
| 56 | t-butyl ester(OtBu) and t-butyl (tBu) |
| 57 | Glycyl (-G-,-Gly-) |
| 57 | Carboxamidomethyl (on Cysteine) |
| 58 | Carboxymethyl (on Cysteine) |
| 60 | sodium + potassium |
| 64 | Selenocysteine (from Serine) |
| 67 | Asp transamidation with piperidine |
| 68 | 3,5-Dichlorination (of Tyrosine with 35Cl) |
| 69 | Dehydroalanine (Dha) |
| 70 | 3,5-Dichlorination (of Tyrosine with mixture of 35Cl and 37Cl)) |
| 70 | Pyruvate |
| 71 | Sarcosyl |
| 71 | Alanyl (-A-, -Ala-) |
| 71 | Acetamidomethyl (Acm) |
| 71 | Propionamide or Acrylamide adduct |
| 72 | 3,5-Dichlorination (of Tyrosine with 37Cl) |
| 74 | S-(sn-1-Glyceryl) (on Cysteine) |
| 74 | Glycerol Ester (on Glutamic acid side chain) |
| 75 | Glycine (G, Gly) |
| 76 | Phenyl ester (OPh) (on acidic) |
| 76 | Beta mercaptoethanol adduct |
| 78 | 3-Bromination (of Tyrosine with 79Br) |
| 78 | L-o-bromination of Phe with 79Br |
| 80 | L-o-bromination of Phe with 81Br |
| 80 | Sulphonation (SO3H) (of PMC group) |
| 80 | Sulphation (of O of Tyrosine) |
| 80 | Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) |
| 80 | 3-Bromination (of Tyrosine with 81Br) |
| 82 | Cyclohexyl ester (OcHex) |
| 83 | Dehydroamino butyric acid (Dhb) |
| 83 | Homoseryl lactone |
| 85 | 2-Aminobutyric acid (Abu) |
| 85 | 2-Aminoisobutyric acid (Aib) |
| 85 | Gamma Aminobutyryl |
| 86 | t-butyloxymethyl (Bum) |
| 86 | Diaminopropionyl |
| 87 | N-(4-NH2-2-OH-butyl)- (of Lysine) (Hypusine) |
| 87 | Seryl (-S-, -Ser-) |
| 88 | t-butylsulfenyl (StBu) |
| 89 | Alanine (A, Ala) |
| 89 | Sarcosine (Sar) |
| 90 | Anisyl |
| 90 | Benzyl (Bzl) and benzly ester (OBzl) |
| 93 | 1,2-ethanedithiol (EDT) |
| 95 | Dehydroprolyl |
| 96 | Trifluoroacetyl (TFA) |
| 97 | N-hydroxysuccinimide (ONSu, OSu) |
| 97 | Prolyl (-P-, -Pro-) |
| 98 | Cysteic acid x2, oxidation of cystine |
| 98 | Tetramethylguanidinium termination by-product on amine |
| 98 | Phosphate/sulphate adduct of proteins |
| 99 | Valyl (-V-, -Val-) |
| 99 | Isovalyl (-I-,-Iva-) |
| 100 | t-Butyloxycarbonyl (tBoc) |
| 101 | Threoyl (-T-, -Thr-) |
| 101 | Homoseryl (-Hse-) |
| 103 | Cystyl (-C-, -Cys-) |
| 104 | 4-Methylbenzyl (Meb) |
| 104 | Benzoyl (Bz) |
| 105 | Serine (S, Ser) |
| 105 | Pyridylethylation of cysteine |
| 106 | HMP (hydroxymethylphenyl) linker |
| 106 | Thioanisyl |
| 106 | Thiocresyl |
| 111 | 2-Piperidinecarboxylic acid (Pip) |
| 111 | Pyroglutamyl |
| 113 | Hydroxyprolyl (-Hyp-) |
| 113 | Isoleucyl (-I-, -Ile-) |
| 113 | Leucyl (-L-, -Leu-) |
| 113 | Norleucyl (-Nle-) |
| 114 | Asparagyl (-N-, -Asn-) |
| 114 | t-amyloxycarbonyl (Aoc) |
| 114 | Ornithyl (-Orn-) |
| 115 | Proline (P, Pro) |
| 115 | Aspartyl (-D-, -Asp-) |
| 117 | Succinyl |
| 117 | Valine (V, Val) |
| 117 | Hydroxybenzotriazole ester (HOBt) |
| 118 | Dimethylbenzyl (diMeBzl) |
| 119 | Threonine (T, Thr) |
| 119 | Cysteinylation |
| 120 | Benzyloxymethyl (Bom) |
| 120 | p-methoxybenzyl (Mob, Mbzl) |
| 121 | 4-Nitrophenyl, p-Nitrophenyl (ONp) |
| 121 | Cysteine (C, Cys) |
| 125 | Chlorobenzyl (ClBzl) |
| 126 | Iodination (of Histidine[C4] or Tyrosine[C3]) |
| 128 | Glutamyl (-Q-, -Gln-) |
| 128 | Lysyl (-K-, -Lys-) |
| 129 | Glutamyl (-E-, -Glu-) |
| 129 | O-Methyl Aspartamyl |
| 130 | N alpha -(gamma-Glutamyl)-Glu |
| 131 | Norleucine (Nle) |
| 131 | Hydroxyproline (Hyp) |
| 131 | Isoleucine (I, Ile) |
| 131 | Leucine (L, Leu) |
| 131 | Methionyl (-M-, -Met-) |
| 131 | Hydroxy Aspartamyl |
| 131 | bb-dimethyl Cystenyl |
| 132 | Asparagine (N, Asn) |
| 132 | Pentoses (Ara, Rib, Xyl) |
| 133 | Aspartic Acid (D, Asp) |
| 134 | Benzyloxycarbonyl (Z) |
| 134 | Adamantyl (Ada) |
| 135 | p-Nitrobenzyl ester (ONb) |
| 137 | Histidyl (-H-, -His-) |
| 142 | N-methyl Glutamyl |
| 142 | N-methyl Lysyl |
| 143 | O-methyl Glutamyl |
| 143 | Diphthamide (from Histidine) |
| 144 | Hydroxy Lysyl (-Hyl-) |
| 145 | Methyl Methionyl |
| 146 | Glutamine (Q, Gln) |
| 146 | Deoxyhexoses (Fuc, Rha) |
| 146 | Lysine (K, Lys) |
| 146 | Pentosyl |
| 146 | Aminoethyl Cysteinyl (AECys) |
| 147 | 4-Glycosyloxy- (pentosyl,C5) (of Proline) |
| 147 | Glutamic Acid (E, Glu) |
| 147 | Phenylalanyl- (-F-, -Phe-) |
| 147 | Methionyl Sulfoxide |
| 148 | Pyridyl Alanyl |
| 149 | 2-Nitrobenzoyl (NBz) |
| 149 | Methionine (M, Met) |
| 149 | Fluorophenylalanyl |
| 150 | Dimethoxybenzyl Trp |
| 153 | 2-Nitrophenylsulphenyl (Nps) |
| 154 | 4-Toluenesulphonyl (Tosyl, Tos) |
| 154 | 3-nitro-2-pyridinesulfenyl (Npys) |
| 155 | Histidine (H, His) |
| 156 | 3,5-Dibromination (of Tyrosine with 79Br) |
| 156 | Arginyl (-R-, -Arg-) |
| 157 | Citrulline |
| 158 | 3,5-Dibromination (of Tyrosine with mixture of 79Br and 81Br) |
| 159 | Dichlorobenzyl (Dcb) |
| 160 | 3,5-Dibromination (of Tyrosine with 81Br) |
| 160 | Carboxyamidomethyl Cystenyl |
| 161 | Hexosamines (GalN, GlcN) |
| 161 | Carboxymethyl cysteine (Cmc) |
| 161 | Carboxymethyl Cystenyl |
| 161 | Methylphenylalanyl |
| 162 | Inositol |
| 162 | N-Glucosyl (N terminus or N epsilon of Lysine) (Aminoketose) |
| 162 | O-Glycosyl- (to Serine or Threonine) |
| 162 | Linker attached to peptide in Fmoc peptide synthesis |
| 162 | Hexoses (Fru, Gal, Glc, Man) |
| 163 | Tyrosinyl (-Y-, -Tyr-) |
| 163 | MethionylSulphone |
| 165 | Phenylalanine (F, Phe) |
| 166 | 2,4 -dinitrophenyl (Dnp) |
| 166 | Pentaflourophenyl (Pfp) |
| 166 | Diphenylmethyl (Dpm) |
| 167 | Phospho Seryl |
| 169 | 2-Chlorobenzyloxycarbonyl (ClZ) |
| 169 | Napthyl acetyl |
| 170 | N-acetyl Lysyl |
| 170 | N-methyl Arginyl |
| 172 | Ethanedithiol/TFA cyclic adduct |
| 173 | Carboxy Glutamyl (Gla) |
| 174 | Arginine (R, Arg) |
| 174 | Acetamidomethyl Cystenyl |
| 174 | Acrylamidyl Cystenyl |
| 176 | N-Glucuronyl (N terminus) |
| 177 | delta-Glycosyloxy- (of Lysine) or beta-Glycosyloxy- (of Phenylalanine or Tyrosine) |
| 177 | 4-Glycosyloxy- (hexosyl,C6) (of Proline) |
| 177 | Benzyl Seryl |
| 177 | N-methyl Tyrosinyl |
| 178 | a-N-Gluconoylation (His Tagged proteins) |
| 179 | p-Nitrobenzyloxycarbonyl (4Nz) |
| 179 | 2,4,5-Trichlorophenyl |
| 180 | 2,4,6-trimethyloxybenzyl (Tmob) |
| 180 | Xanthyl (Xan) |
| 181 | Tyrosine (Y, Tyr) |
| 182 | Chlorophenylalanyl |
| 182 | Mesitylene-2-sulfonyl (Mts) |
| 183 | AEBSF |
| 184 | Isopropyl Lysyl |
| 186 | Tryptophanyl (-W-, - Trp-) |
| 186 | Carboxymethyl Lysyl |
| 188 | N-Lipoyl- (on Lysine) |
| 190 | Matrix alpha cyano MH+ |
| 191 | Benzyl Threonyl |
| 193 | Benzyl Cystenyl |
| 197 | Napthyl Alanyl |
| 198 | Succinyl Aspartamyl |
| 201 | HMP (hydroxymethylphenyl)/TFA adduct |
| 203 | N-acetylhexosamines (GalNAc, GlcNAc) |
| 204 | Tryptophan (W, Trp) |
| 204 | Cystine ((Cys)2) |
| 204 | Farnesylation |
| 206 | S-Farnesyl- |
| 206 | Myristoylation-4H (2 double bonds) |
| 208 | Myristoleylation (myristoyl with one double bond) |
| 208 | Pyridylethyl Cystenyl |
| 210 | Myristoylation |
| 212 | 4-Methoxy-2,3,6-trimethylbenzenesulfonyl (Mtr) |
| 213 | 2-Bromobenzyloxycarbonyl (BrZ) |
| 214 | Formyl Tryptophanyl |
| 219 | Benzyl Glutamyl |
| 219 | Anisole Adducted Glutamyl |
| 222 | 9-Fluorenylmethyloxycarbonyl (Fmoc) |
| 222 | S-cystenyl Cystenyl |
| 226 | Biotinylation (amide bond to lysine) |
| 226 | Dimethoxybenzhydryl (Mbh) |
| 229 | N-Pyridoxyl (on Lysine) |
| 231 | Pyridoxal phosphate (Schiff Base formed to lysine) |
| 233 | Dansyl (Dns) |
| 233 | Nicotinyl Lysyl |
| 238 | 2-(p-biphenyl)isopropyl-oxycarbonyl (Bpoc) |
| 238 | Palmitoylation |
| 242 | Triphenylmethyl (Trityl, Trt) |
| 243 | Tyrosinyl Sulphate |
| 243 | Phospho Tyrosinyl |
| 252 | Pbf (pentamethyldihydrobenzofuransulfonyl) |
| 252 | 3,5-Diiodination (of Tyrosine) |
| 258 | a-N-6-Phosphogluconoylation (His Tagged proteins) |
| 259 | N alpha -(gamma-Glutamyl)-Glu2 |
| 266 | O-GlcNAc-1-phosphorylation (of Serine) |
| 266 | Stearoylation |
| 266 | Pmc (2,2,5,7,8- Pentamethylchroman-6-sulphonyl) |
| 272 | Geranylgeranylation |
| 272 | Monomethoxytrityl |
| 276 | S-Geranylgeranyl |
| 289 | 5'phos dCytidinyl |
| 289 | iodo Tyrosinyl |
| 290 | Aldohexosyl Lysyl |
| 291 | Sialyl |
| 291 | N-acetylneuraminic acid (Sialic acid, NeuAc, NANA, SA) |
| 304 | 5'phos dThymidinyl |
| 305 | 5'phos Cytidinyl |
| 305 | Glutathionation |
| 306 | O-Uridinylylation (of Tyrosine) |
| 306 | 5'phos Uridinyl |
| 307 | N-glycolneuraminic acid (NeuGc) |
| 307 | S-farnesyl Cystenyl |
| 313 | 5'phos dAdenosyl |
| 324 | O-pantetheinephosphorylation (of Serine) |
| 327 | SucPhencarb Lysyl |
| 329 | 5'phos dGuanosyl |
| 329 | 5'phos Adenosinyl |
| 329 | O-5'-Adenosylation ( of Tyrosine) |
| 339 | 4'-Phosphopantetheine |
| 342 | S-palmityl Cystenyl |
| 345 | 5'phos Guanosyl |
| 354 | Biotinyl Lysyl |
| 359 | Fluorescein labelling of peptide N-terminal using NHS ester |
| 365 | Hex-HexNAc |
| 388 | N alpha -(gamma-Glutamyl)-Glu3 |
| 391 | Dioctyl Phthalate |
| 395 | PMC Lysyl |
| 409 | Aedans Cystenyl |
| 413 | Dioctyl Phthalate Sodium Adduct |
| 415 | di-iodo Tyrosinyl |
| 423 | PMC Arginyl |
| 454 | S-Coenzyme A |
| 457 | AMP Lysyl |
| 470 | 3,5,3'-Triiodothyronine (from Tyrosine) |
| 524 | S-(sn-1-Dipalmitoyl-glyceryl)- (on Cysteine) |
| 541 | S-(ADP-ribosyl)- (on Cysteine) |
| 541 | N-(ADP-ribosyl)- (on Arginine) |
| 541 | O-ADP-ribosylation (on Glutamate or C terminus) |
| 541 | ADP-rybosylation (from NAD) |
| 587 | S-Phycocyanobilin (on Cysteine) |
| 617 | S-Heme (on Cysteine) |
| 648 | N theta -(ADP-ribosyl) diphthamide (of Histidine) |
| 657 | NeuAc-Hex-HexNAc |
| 783 | O-8 alpha-Flavin [FAD])- (of Tyrosine) |
| 784 | S-(6-Flavin [FAD])- (on Cysteine) |
| 784 | N theta and N pi-(8alpha-Flavin) (on Histidine) |
| 893 | (Hex)3-HexNAc-HexNAc |
| 1,039 | (Hex)3-HexNAc-(dHex)HexNAc |
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'이전 바이오텍 카테고리/MolBiol Programs'의 다른글
- 현재글MASS로부터 단백질의 post-tanslational modification 예측하기