Microbial production of fatty-acid-derived fuels and chemicals from plant biomass Eric J. Steen, Yisheng Kang, Gregory Bokinsky, Zhihao Hu, Andreas Schirmer, Amy McClure, Stephen B. del Cardayre & Jay D. Keasling Nature 463, 559-562(28 January 2010)
이 논문의 의의는 바이오연료라 함은 바이오에탄올과 바이오디젤이 양대 산맥인데 바이오디젤은 지방 함량이 높은 식물을 주로 이용해 왔고 바이오에탄올은 탄수화물(옥수수의 전분질, sugarcane의 설탕, 셀룰로스나 헤미셀룰로스)등을 이용해왔죠. 그런데 이번 논문은 탄수화물을 이용해서 바이오디젤을 만드는 방법이라는 것입니다.
Flux through the E. coli fatty acid pathway (black lines) was increased to improve production of free fatty acids and acyl-CoAs by eliminating β-oxidation (knockouts are fadE), by overexpressing thioesterases (TES) and acyl-CoA ligases (ACL). Various products were produced from non-native pathways (orange lines) including biodiesel, alcohols and wax esters. Alcohols were produced directly from fatty acyl-CoAs by overexpressing fatty acyl-CoA reductases (FAR); the esters were produced by expressing an acyltransferase (AT) in conjunction with an alcohol-forming pathway; biodiesel was produced by introduction of an ethanol pathway (pdc and adhB) and wax esters were produced from the fatty alcohol pathway (FAR). Finally, expressing and secreting xylanases (xyn10B and xsa) allowed for the utilization of hemicellulose. Overexpressed genes or operons are indicated; green triangles represent the lacUV5 promoter. AcAld, acetaldehyde; EtOH, ethanol; pyr, pyruvate.
대장균 유전자 재조합 방법 (EJ Steen et al. Nature 463, 559-562 (2010) doi:10.1038/nature08721)
그놈의 impact factor 4, 5도 안되는 AEM 논문만 왕창쓴다고 무시하던(?) 사람들, 이젠 왜 Keasling이 합성생물학의 대가인지 알게 되었을 것으로 생각합니다. (그러니까 그놈의 impact factor 타령 좀 그만하자구요.)